Rabies virus inactivation by binary ethylenimine: new method for inactivated vaccine production

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Rabies virus inactivation by binary ethylenimine: new method for inactivated vaccine production.

The inactivation dynamics of rabies virus (PV strain) by binary ethylenimine, and the immunogenic properites and the stability of the vaccines prepared using this agent, were studied. Binary ethylenimine at a final concentration of 0.01 M was prepared wtih 2-bromoethylamine hydrobromide in alkaline solutions, either separately from or in suspensions of rabies virus propagated in BHK cells. The ...

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Ethylenimine-inactivated rabies vaccine of tissue culture origin.

The replication of seven rabies virus strains (CVS, HEP, PV, ERA, WIRAB, CPZ and BOLIVAR) in BHK cells and the inactivation dynamics of these strains by beta-propiolactone, acetylethylenimine, and ethylenimine were studied to find the most immunogenic strain and the most economic and stable inactivating agent for the production of an inactivated tissue culture rabies vaccine for animal use. The...

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Immunogenicity of commercial, formaldehyde and binary ethylenimine inactivated Newcastle disease virus vaccines in specific pathogen free chickens

Newcastle disease (ND) is one of the most important diseases that affect birds; the epizootic nature of the disease has caused severe economic losses in the poultry industry worldwide. In this experiment ND virus (NDV) was inactivated by two different chemicals binary ethylenimine (BEI) and formaldehyde. Formaldehyde was used at 0.1%, while BEI was used at concentrations of 1 to 4 mM. NDV inact...

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Immune Response to Inactivated Newcastle Disease Virus by Electrolysed Catholyte Anolyte and Binary Ethylenimine in Specific Pathogen Free Chickens

This study aimed to investigate the immunogenicity of Newcastle disease virus (NDV) in specificpathogen-free (SPF) chickens. The NDV was inactivated using either Binary ethyleneimine (BEI) or Electrolysed water-Catholyte-Anolyte (ECA). Complete inactivation of NDV occurred after 24 hours with either BEI or ECA. Prepared inactivated NDV vaccines were tested for their efficiency in generating hum...

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 1980

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.11.2.120-122.1980